Study on differential genes expression in HBV-related heptocellular carcinoma
- VernacularTitle:HBV相关肝细胞癌的基因差异表达
- Author:
Yukun HUANG
;
Xuegong FAN
;
Fu QIU
;
Jiang LI
;
- Publication Type:Journal Article
- Keywords:
LIVER NEOPLASMS/pathol;
HEPATOMA/pathol;
GENES EXPRESSION;
ONCOGENES;
TUMOR SUPPESSOR GENES
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of oncogenes and tumor suppressor genes in hepatitis B virus (HBV) related hepatocellular carcinoma (HCC). Methods mRNA was extracted from cancerous and paracancerous tissues of 22 patients with HBV related HCC and synthesized into cDNA. The cDNA labeled with 33p dATP was hybridized for cDNA microarray each consisting of 3170 genes or expressed sequence tags (EST). The differential expression genes were searched in gene data base and verified using RT PCR. Results The differential expression of 1369 genes or EST was identified including 121 genes or EST altered 2 times or more in cancerous tissues compared with paracancerous tissues. Compared with paracancerous tissues, 88 showed overexpression and 33 genes were down regulated. The positive expression of transmembrane 4 superfamily member 1 (TM4SF1) in cancerous tissues was 86.4 % and could not be detected in paracancerous tissues (P
