Effect of endothelin-1 monoclonal antibody on apoptosis of hepatocytes during ischemia/reperfusion injury of liver transplantation in rats
- VernacularTitle:内皮素1单抗对肝移植缺血再灌注损伤中肝细胞凋亡的影响
- Author:
Zhiheng HUANG
;
Chengyou WANG
;
Yong NI
;
Minjie ZHANG
;
Guihua CHEN
;
- Publication Type:Journal Article
- Keywords:
LIVER TRANSPLANTATION;
APOPTOSIS;
ISCHEMIA REPERFUSION INJURY;
ENDOTHELIN-1 MONOCLONAL ANTIBODY
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of hepatocyte apoptosis in ischemia/reperfusion injury of grafted liver and the effects of endothelin 1 (ET 1) monoclonol antibody on hepatocyte apoptosis.Methods Othotopic liver transplantation rats were divided into two groups: with and without ET 1 antibody. The concentrations of ET 1 of plasma and liver tissue were measured. The parameters of liver fuction were determined . The concentration of malondialdehyde (MDA) was measured and the number of apoptotic hepatocytes in grafts liver was determined.Results The levels of ET 1, ALT in serum, and ET 1, MDA and apoptotic cells in the grafted liver after ischemia/reperfusion were significantly increased compared with normal values. In the ET 1 antibody group, the levels of ET 1, ALT, MDA and apoptotic cells were significantly decreased. Conclusions ET 1 monoclonal antibody can attenuate ischemia/reperfusion injury by decreasing lipid peroxide reaction, and decreasing apoptosis of hepatocytes and thus protect the liver graft.
