Significance of TLR2 expression in ischemic liver tissue after liver ischemia/reperfusion in mice
- VernacularTitle:小鼠肝缺血再灌注后Toll样受体2在缺血肝组织中的激活及其意义
- Author:
Jinxiang ZHANG
;
Heshui WU
;
Hui WANG
;
Yang WANG
;
Yuan TIAN
;
Qichang ZHENG
- Publication Type:Journal Article
- Keywords:
LIVER ISCHEMIA REPERFUSION INJURY;
TLR2;
LIVER FUNCTION
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore Toll like receptor 2 (TLR2) expression after partial liver ischemia/ reperfusion (I/R) in BALB/c mice and its relationship with liver function impairment. Methods All the animals were randomly divided into ischemia/reperfusion injury (I/R) and sham operation (S) groups. Total RNA was extracted from the liver samples and the expression of TLR2 mRNA was analyzed quantitatively by real time PCR method. Membrane protein was extracted, and the expression of TLR2 protein was detected by western blot method. Portal vein serum and plasma were taken for further detection of the levels of alanine aminotransferase (ALT), tumor necrosis factor alpha (TNF ?) and endotoxin . Results After 1h of partial liver ischemia and 4 h of reperfusion, the expression of TLR2 mRNA and TLR2 protein were both remarkably upregulated in tissues of ischemic liver lobes in I/R group compared with that in S group (value of TLR2mRNA: 1.06?0.91vs5.08?1.32,P0.05). Conclusions TLR2mRNA and TLR2 protein expression were upregulated in tissues of ischemic liver lobes after partial I/R injury in mice, and were associated with increased levels of portal vein ALT and TNF ?,and impairment of liver function.
