Effects of Antisense NF-?B p65 Oligodeoxynucleotides on UV-induced IL-6 Secretion of Keratinocytes
- VernacularTitle:核因子-?B反义寡核苷酸抑制紫外线诱导的HaCaT细胞反义分泌白介素6
- Author:
Jiping XIA
;
Xiuzu SONG
;
Zhigang BI
;
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
Keratinocytes;
NF-kappa B;
Oligonucleotides, antisense
- From:
Chinese Journal of Dermatology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of antisense NF-?B p65 oligodeoxynucleotides on UV-induced IL-6 secretion of keratinocytes. Methods NF-?B p65 oligodeoxynucleotides were transfected to a keratinocyte cell line, HaCaT cells, mediated by lipofectamine. The NF-?B p65 protein in the cells was measured by Western blot assay, the mRNA level of NF-?B p65 was detected by RT-PCR, and UV-induced IL-6 level was determined by ELISA pre- and post-transfection and/or UVB irradiation. Results The NF-?B p65 protein expression was significantly increased in UVB (10, 20, 30 mJ/cm2 ) irradiation groups as compared with that of the control groups (P
