Effects of Cryptotanshinone and Tanshinone ? on Proliferation, Lipid Synthesis and Expression of Androgen Receptor mRNA in Human Sebocytes in vitro

VernacularTitle:丹参酮对人皮脂腺细胞增殖、脂质合成及雄性激素受体mRNA表达的影响
Author: Qiang JU ; Xingping YIN ; Jihai SHI ; Yan XIN ; Xiaojing KANG ; Yun CHEN ; Pangen CUI ; Yuanhua CAO ; Longqin XIA ;
Publication Type:Journal Article
Keywords: Cryptotanshinone; Tanshinone; Sebaceous glands; Cells, Cultured; Lipid; Receptor, androgen
From: Chinese Journal of Dermatology 1994;0(02):-
CountryChina
Language:Chinese
Abstract: Objective To study the effects of tanshinone on the cultured immortalized human sebocytes and explore the mechanism of action of tanshinone in the treatment of acne. Methods MTT assay was applied to determine the effects of cryptotanshinone and tanshinone Ⅱ? at different concentrations on the proliferation of SZ95 sebocytes in 24 h, 48 h and 72 h after incubation. Lipid contents labeled with Nile red in SZ95 cells were analyzed by flow cytometry technique, and the expression of androgen receptor (AR) mRNA of SZ95 cells were detected by RT-PCR. Results Both cryptotanshinone and tanshinoneⅡ? inhibited the proliferation of SZ95 cells in a dose- and time-dependent mode, with the 50% inhibition concentration (IC50) of 7.473 ?mol/L (in 48 h) and 2.146?mol/L (in 72 h) for cryptotanshinone and 6.021 ?mol/L (in 48 h) and 2.25 ?mol/L (in 72 h) for tanshinone Ⅱ?. Additionally, as compared with the control group, the lipid content of SZ95 cells exposed to tanshinone Ⅱ? at 0.125?mol/L was decreased in 48h (P