Experimental study on myocardial injury and protective effect of ulinastatin after bile duct obstruction
- VernacularTitle:梗阻性黄疸对心肌的损伤及乌司他丁保护作用的研究
- Author:
Mingde ZHU
;
Chihua FANG
;
Tiejun CHEN
;
- Publication Type:Journal Article
- Keywords:
CHOLESTASIS/compli;
MYOCARDIAL INJURY/etiol;
MYOCARDIUM/pathol;
ULINASTATIN/ther use
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the myocardial injury induced by bile duct obstruction and the protective effects ulinastatin(UTI). Methods Dynamic observation of of the levels of malondialdyhyde(MDA) and superoxide dismutase(SOD) of myocardial tissues, and the levels of serum TBil, alkaline phosphatase(ALP), MB isoenzyme of creatine kinase(CK MB), endotoxin (ET), and tumor necrosis factor(TNF ?) in bile duct obstruction(BLD) rats and UIT treatment rats. The left ventricles of the rats were obtained for light and electronic microscopic observation . Immunohistochemical staining method of ABC used was to locate the expression and distribution of TNF ? in myocardial tissues. Results After ligation of the common bile duct, serum TBIL, ALP, CK MB, ET, TNF ? levels and myocardium MDA gradully increased, while SOD levels gradually decreased, and the expression of TNF ? in myocardium increased. As compared with BDL group at the same phase, in UIT group, serum TBIL, ALP, CK MB, ET, TNF? levels and myocardium MDA in UTI treated groups decreased, while myocardium SOD increased, and the expression of TNF ? in myocardium decreased.Myocardial injuries of bile duct obstruction were aggravated as time progressed, and there were less myocardial injuries in UTI treated groups than in BDL groups at the same stage as shown with light and electronic microscopic observation.Conclusions UTI can effectively protect the myocardium from ET,TNF ? and free radical injury in bile duct obstucion rats.
