Study of differentially expressed genes in tamoxifen resistance breast cancer cell line LCC2 by cDNA microarray
- VernacularTitle:乳腺癌激素耐受细胞系LCC2的基因表达分析
- Author:
Jianying CHEN
;
Bo ZHANG
;
Guobin WANG
;
Daoda CHEN
;
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Gene expression;
DNA microarray
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for the differentially expressed genes in breast cancer cell line MCF 7 and tamoxifen resistant breast cancer cell line LCC2 by using cDNA microarray Methods The PCR products of 8000 genes were spotted on chemical material coated glass plates in array The DNAs were then fixed on the glass plate by a serial of treatments The total RNAs were isolated from cells cultured in the flash, and then were purified to mRNA by Oligotex Both the mRNAs from different breast cancer cell lines were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP, for preparing the hybridization probes The mixed probes were then hybridized to the cDNA microarray After high stringent washing, the cDNA, microarray was scanned for the fluorescent signals and showed the differences between the cell lines Results Among the 8?000 target genes, there were 1?892 (23 65%) genes expressing differently between MCF 7 and LCC2 cell lines Bioinformational analysis on those genes was performed Conclusion DNA microarray technology is an effective technique in screening for differentially expressed genes between different tissues and cell lines
