Effect of ursodeoxycholic acid on visceral yolk sac cell membrane fluidity and placental glutathione concentration in pregnant rats with intrahepatic cholestasis
- VernacularTitle:熊去氧胆酸对肝内胆汁淤积孕鼠卵黄囊细胞膜流动性及胎盘组织谷光甘肽含量的影响
- Author:
Qingyun SHI
;
Beihua KONG
;
Peng LI
;
Kaidong MA
;
Sen JIANG
;
- Publication Type:Journal Article
- Keywords:
Ursodeoxycholic acid;
Cholestasis, intrahepatic;
Vitelline membrane;
Membrane fluidity;
Glutathione
- From:
Chinese Journal of Perinatal Medicine
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of ursodeoxycholic acid (UDCA) on the fluidity of rat visceral sac and placental glutathione (GSH) concentration in rats with intrahepatic cholestasis. Methods Sixty rats were randomly divided into 3 groups (20 in each). Refined vegetable oil 2.5 ml/(kg?d) was given to the control group since the 13 days of pregnancy. The ICP treatment and non treatment group received either progesterone 75 mg/(kg?d) or 17? ethynylestradiol 1.25 mg/ (kg?d) from the 13th to 17th day, respectively. From the 17th day, the control and non treatment group were fed with 0.9% nitrachloride solution 5 mg/(kg?d) and the treatment group with UDCA 50 mg/(kg?d). All rats were sacrificed on the 21st day. The visceral yolk sac cell membrane and GSH concentration were measured Results The concentration of GSH in the ICP non treatment group (1.12?0.02 mmol/g protein) was significantly lower than that of the treatmentgroup (1.38?0.03 mmol/g protein) and the control group (1.56?0.07 mmol/g protein) ( P 0.05). The fetal death rate in treatment group (9.55%) and control group (1.97%) was significantly lower than that of the non treatment group (20.47%) ( P
