Research on the changes of expression of MMP 2 and TIMP 2 in the neonatal rats lung tissue caused by LPS
- VernacularTitle:内毒素对新生大鼠肺组织金属基质蛋白酶2及其抑制因子2表达影响的研究
- Author:
Yue DU
;
Xuxu CAI
;
Yubin WU
;
Hong GAO
;
Yukun HAN
;
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Rats;
Lung;
Hemorrhage;
Gelatinase A;
Tissue inhibitor of metalloproteinase 2
- From:
Chinese Journal of Perinatal Medicine
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of metalloproteinase 2 (MMP 2) and tissue inhibitor of metalloproteinase 2 (TIMP 2) in neonatal acute lung injury(ALI) caused by LPS in rats. Methods Eighty eight newborn rats of 7 days were randomly divided into eight groups: control, 0.5 h, 1 h, 2 h, 4 h, 8 h, 16 h, 24 h after LPS was injected. The changes of lung pathology in newborn rats were observed and the changes of MMP 2 and TIMP 2 expression were measured by immunohistochemistry and RT PCR. Results Pulmonary hemorrhage was seen in newborn rats caused by LPS. The expression of MMP 2 mRNA and protein were 0.523?0.030 and 126.20?17.98; The expression of MMP 2 mRNA increased and the highest level was at 4 h(0.826?0.567, t=3.77, P
