Effects of culture medium of amniotic cells on NO and NOS in retina tissues in vitro
- VernacularTitle:羊膜细胞培养液对体外培养视网膜组织中一氧化氮及合成酶的影响
- Author:
Rui-Zhuang LI
;
Xuan-Wei LIANG
;
Can-Rong YAN
;
Hai-Lan LIAO
;
Hong-Ni SONG
;
Biao ZHENG
- Publication Type:Journal Article
- Keywords:
Retina/lenzymology;
Nitros oxide;
Nitric-oxide synthase;
Cell culture;
Amnions
- From:
Chinese Journal of Ocular Fundus Diseases
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of culture medium of a mn iotic cells on NO and NOS in retinal tissues of rabbits in vitro in order to pro vide a protective method for antioxidation in retina transplantation. M ethods Thirty adult healthy rabbits (30 right eyes) were divided into 3 groups. Group Ⅰ: fresh retinal tissue; group Ⅱ: routine culture medium; group Ⅲ: culture medium of amniotic cells. The retinal tissues in group Ⅱ and Ⅲ we re cultured in the corresponding culture medium for 1 week. The content of NO an d NOS in retinal tissues in the 3 groups were determined. Results Compared with group I, the content of NO and NOS of group Ⅱ increased obvio usly (t=3.821, 3.854; P0.05) . Conclusion Culture medium of amniotic cells may remove free r adicals and enhance the ability of antioxidation.
