Apoptosis induced by cold ischemia and reperfusion after pancreas transplantation in rats
- VernacularTitle:大鼠移植胰腺冷缺血再灌注后细胞凋亡及其调控基因的研究
- Author:
Xi LI
;
Zhaohui ZHANG
;
- Publication Type:Journal Article
- Keywords:
Pancreas transplantation;
Reperfusion injury;
Apoptosis;
Gene expression
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate apoptosis in acinar cells during posttransplant reperfusion, and the expression of Bcl 2, Bax, Fas Methods Pancreas transplantation was performed between male SD rats after 2 hours of cold ischemia Rats ( n =65) were divided at random into 7 groups: sham group, cold ischemia group (as controls), reperfused 1 h, 3 h, 6 h, 9 h, 12 h group The pathologic pancreatic tissue was observed by electron microscope; Apoptotic cells were stained by TUNEL technique; Expression of Bax、Bcl 2 and Fas protein in pancreatic tissue were detected by SABC immunohistochemical method Results Apoptotic cells were observed in early phase after pancreas transplantation The peak of apoptotic pancreas cells occurred 3 hours after reperfusion [AI ( 9 5? 2 9)%, P
