An experimental study of gene therapy targeted by magnetic nanosphere for occlusive vascular disease
- VernacularTitle:纳米磁粒靶向基因治疗动脉闭塞性疾病的实验研究
- Author:
Tiemin ZHANG
;
Jun XU
;
Hongchi JIANG
;
Jinpeng ZHAO
;
Lanlan WEI
;
Min ZHUANG
;
Xiulin CHEN
;
Hongxi GU
;
- Publication Type:Journal Article
- Keywords:
Arterial occlusivev diseases;
Endothelial growth factors;
Endothelium, vascular;
Genes
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate a new gene therapy for the treatment of experimental occlusive arterial disease Methods Magnetic nanospheres were produced, VEGF gene was cloned for subsequent construction of eukaryotic expression plasmid The magnetic gelatin microspheres used in targeted gene therapy were prepared by emulsion crosslinking method The microspheres were injected intrafemorally in rabbits through contralateral femoral artery, and the ischemic limb was placed in a magnetic field Angiography was performed on day 10 and day 30 respectively The capillary density and the capillary to muscle fiber ratio were determined histochemically Results Compared to the controls there was significant collateral artery development in VEGF transfected group The capillary density and the capillary to muscle fiber ratio were significantly higher for the VEGF transfected group than for the control group. The capillary density of control was (125?23)/mm 2, and in VEGF group was (298?27)/mm 2, P
