Changes of intercelluar adhesion molecule-1 and inhibitor of nuclear factor ?B after lipopolysaccharide induced pulmonary hemorrhage in neonatal rats
- VernacularTitle:脂多糖致新生大鼠肺出血、肺组织细胞间黏附分子1和核因子?B抑制蛋白表达的变化
- Author:
Xuxu CAI
;
Yue DU
;
Yunxiao SHANG
;
ETAL
- Publication Type:Journal Article
- Keywords:
Intercelluar adhesion molecule 1;
NF-kappa B;
Lipopolysaccharides;
Lung diseases;
Hemorrhage;
Rats
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes and effects of intercelluar adhesion molecule-1 (ICAM-1) and inhibitor protein of nuclear factor-?B(I?B?) in pulmonary hemorrhage induced by endotoxin lipopolysaccharide (LPS) in neonatal rats. Methods Sixty-eight seven to ten-day-old Wistar rats were separated into 2 groups randomly. In the experimental groups, LPS was injected intraperitoneally at the dosage of 5 mg/kg and the normal saline (NS) control group was injected with equal amount of NS intraperitoneally. The rats were sacrificed at 30 min, 1, 2, 4, 8, 16 and 24 h post injection. The histology of lung tissues were examined by microscope and electron microscope. The expression of ICAM-1, I?B?protein and mRNA expression were observed using Western blot and in situ hybridization. Results One hour after LPS administration, pulmonary hemorrhage and inflammatory responses were observed in the rat lungs. The ICAM-1 protein expression greatly increased at 2 h post injection and it was significantly higher than control group(178.13?8.18 vs 161.57?12.52, P
