Inducible nitric oxide synthase and nitric oxide in smooth muscle cells of human abdominal aortic aneurysm
- VernacularTitle:体外培养的人腹主动脉瘤血管平滑肌细胞中一氧化氮的生成和诱生型一氧化氮合酶的表达
- Author:
Mingfang LIAO
;
Zaiping JING
;
Xin ZHAO
;
Suzhen ZHANG
;
- Publication Type:Journal Article
- Keywords:
Aortic aneurysm, abdominal;
Nitric oxide;
Nitric-oxide synthase;
Smooth muscle cell
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide(NO) in cultured vascular smooth muscle cells(SMCs)from human abdominal aortic aneurysm(AAA). Methods TypeⅠ collagenase enzyme digestion method was used to culture AAA SMC . Cell proliferation curves for AAA SMC proliferation ability were evaluated,? smooth muscle actin (? SMA) , iNOS protein expression of AAA SMC and nitrite and nitrate (NO 2 -+NO 3 -,NO X ) in cultured media were measured. Results AAA SMCs had a limited proliferation ability, with 45%?5 8% positive rate of ? SMA and 86 7%?4 6% of iNOS protein expression. High concentration of NO X existed in the cultured media. Conclusion AAA SMCs present abnormal but limited proliferation and changed phenotype; The expression of iNOS and high production of NO of AAA SMCs may play an important role in AAA pathogenesis.
