Micro satellite instability in the development of gastric cancer
- VernacularTitle:微卫星不稳定性与胃癌发生关系的研究
- Author:
Xiaoyong ZHANG
;
Ping LIU
;
Yun SHAO
;
- Publication Type:Journal Article
- Keywords:
Micro satellite instability;
Precancerous conditions;
Stomach neoplasm
- From:
Chinese Journal of Digestive Endoscopy
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the micro satellite instability (MSI) from the precancerous and cancerous lesions (GC) and its effect on carcinogenesis.Methods Silver staining single strand conformation polymorphism polymerize chain reaction (PCR SSCP) was used to screen MSI markers at 5 loci in formalin fixed,paraffin embedded tissues of GC (n=30), dysplasia (n=30), intestinal metaplasia (IM) ( n =40) and corresponding normal gastric tissues.Results The abnormal shifting of the single strand DNA was identified in 7(23 3%)out of GC, in 9 (30%) out of dysplasia and in 8(20%) out of IM samples respectively. GC with MSI was associated with distal location of the tumors ( P =0 044). MSI was not detected in low grade IM tissues.Conclusion MSI may be of an early event during gastric carcinogenesis. It contributes probably to the acquisition of a transformed cell phenotype and to the development of gastric cancer.
