Role of gelatinase and interleukin-8 in the pathogenesis of premature rupture of membrane
- VernacularTitle:羊水中明胶酶、白细胞介素8与胎膜早破关系的研究
- Author:
Jianxin GUO
;
Li LI
;
Zhuqin CHEN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Gelatinases;
Fetal membranes, premature rupture;
Interleukin 8;
Amniotic fluid
- From:
Chinese Journal of Perinatal Medicine
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of Gelatinase and interleukin 8 (IL 8) in premature rupture of membrane (PROM). Methods Sixty pregnant women were divided into two groups. In the observation group, were the pregnant women ( n=30 ) complicated with PROM. The women whose membranes ruptured either normally or manually in cesarean delivery were in the control group. Activities of total gelatinases in amniotic fluid were calculated after the experiment of enzyme linked immunosorbent assay (ELISA) with actin labeled gelatin. The electrophoresis of gelatinase was taken according to reference. IL 8 was determined by sandwich ELISA. Results (1) The activity of total gelatinases of amniotic fluid in observation and control groups during the period before labor, latent stage and active stage were (85?10)?g/L?12 h, (91?7)?g/L?12 h, (104?23)?g/L?12 h, (49?15)?g/L?12 h, (58?15)?g/L?12 h and (102?29)?g/L?12 h respectively,the activity of total gelatinases in active stage was significantly higher than that before labor ( P
