Analysis of diazepam and its main metabolites in urine by GC-ECD
- VernacularTitle:尿中地西泮及其代谢物的气相色谱电子捕获检测法分析
- Author:
Dongxian LIU
;
Jiangnan HE
;
- Publication Type:Journal Article
- Keywords:
Forensic toxicological;
Diazepam;
Matabolites;
Gas chromatography;
Urine
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A method was developed for the determination diazepam and its main metabolites,nordiazepam, and oxazepam and then in urine by GC-ECD. Method The urine samples were hydrolyzed with ?-glucuronidase were extracted in organic solvent. The extractives were derived with BSTFA and the analytes as trimethylsilyl derivatives were determined by GC. Results Sensitive of the method is as low as snglme, the recovery is high then 70%. Conclusion This method is sensitive enough for the analysis of urine from the subjects over a 48 hour period after receiving a 10mg dose of diazepam orally.
