The expression of ICAM-1 and P-selectin in the healing process of incised wounds of rats
- VernacularTitle:大鼠皮肤切创愈合过程中ICAM-1及P选择素的表达
- Author:
Zhiming CHEN
;
Dewen WANG
;
Xu WU
;
Guohua ZHANG
;
Yu DU
;
Wei ZHANG
;
Hongshi CAO
;
Chang LI
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Skin injury;
ICAM-1;
P-selectin;
Immunohistochemistry;
Timing of wound age
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Explore the expression of intercellular adhesion molecule-1(ICAM-1) and Pselectin in the healing process for possible application in timing of incised wounds. Method An immunohistochemical study on the expression of ICAM-1 and P-selectin was performed on rats vital skin incised wounds (5min～7d)using postmortem incisions(5～30min)as control.Results In the vital skin incisions,positive stain of ICAM-1 was observed on epidermis began from 1h and lasted till 3d, while the P-selectin stain was observed on vascular endothelium as early as 10min after incision and lasted 5h; in addition, the expression of ICAM-1 was also found in the inflammatory cells and fibroblasts,the lasting period varied from group to group (groupⅠ 5min～1h,groupⅡ 3～7h,group Ⅲ 9～12h,groupⅣ 1d,groupⅤ 3d,groupⅥ 5～7d.).The positive ratios of ICAM-1 among the cells were very low in group Ⅰ(0.41?0.73%),and increased considerably in group Ⅱ(9.79?3.74%) and group Ⅲ(23.33?1.10%),maximized in group Ⅳ(30.58?2.65%),then decreased in group Ⅴ and Ⅵ.There were no changes of ICAM-1 and P-selectin in the postmortem incision group. Conclusion ICAM-1 and P-selectin are considered potentially useful markers for wound age determination in forensic practice.
