Immunohistochemical study of endothelin in early acute myocardial ischemia in rats
- VernacularTitle:大鼠急性心肌缺血早期内皮素的免疫组化染色观察
- Author:
Zhiyong WANG
;
Zheng LI
;
Xinshan CHEN
;
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Endothelin;
Immunohistochemistry;
Early myocardial ischaemia;
Sudden coronary death
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study the expression of endothelin(ET) of ischaemic myocardium in rat. Method Sixty Wistar rats were randomly divided into 5 groups: coronary artery simply-ligated group,drug-pre-treated group(Isoproterenol,Pituitrin),coronary atherosclerotic group,sham-operated group(as control group). The samples were cut at 15min,30min,45min,60min,90min,120min after coronary (left anterior descending) ligation, and stained with HE and strept avidin-biotin enzyme complex(SABC)method. Results The expression of endothelin was observed with light microscope. Ischaemic myocardium showed ET-positive staining at the early stage of acute myocardial ischaemia in different groups and more in the pre-treated group. Conclusion the SABC-ET method may provide a new marker for diagnosing early myocardial ischaemia, which will be helpful in confirming the cause of sudden coronary death(SCD).
