Changes of the levels of tumor necrosis factor and interleukin-8 in the blood and gastric mucosa and their mRNA expression after liver ischemia- reperfusion
- VernacularTitle:肝缺血再灌注对血及胃黏膜中TNF和IL-8及其mRNA表达的影响
- Author:
Yankui CHU
;
Qingjiu MA
;
Wei LIU
;
Jiangou LU
;
Xilin DU
;
Deming GAO
;
Shengzhi WANG
;
Tao WU
- Publication Type:Journal Article
- Keywords:
LIVER/blood supply;
REPERFUSION INJURY;
GASTRIC MUCOSA/pathol;
TUMOR NECROSIS TACTOR;
IMTERLUKIN-8;
GENE EXPRESSION
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of the levels of TNF and IL 8 in blood and in gastric mucosa and their mRNA expression after liver ischemia reperfusion (I/R). Methods 130 rats were randomly divided into 3 groups: (1) sham operation group(control group, n=10);(2)liver ischemia reperfusion group (n=60), including ischemia for 20min and 40min, and reperfusion for 1h, 24h and 72h respectively; (3) liver I/R rats treated with famotidine group (n=60). ALT and AST were detected after operation, pathomorphological changes of gastric mucosa were studied by light microscope and transmission electronic microscope, the concentrations of TNF and IL 8 in blood and gastric mucosa were measured by radioimmunoassay (RIA), TNF mRNA and IL 8 mRNA were detected by in situ hybridization. Results After liver I/R,inflammatory damage appeared in gastric mucosa, TNF and IL 8 concertrations increased significantly(P
