Effect of raised intracellular free calcium on the expression of Caspase-3,Bax and Bcl-2 in human breast cancer MCF- 7 cell lines
- VernacularTitle:钙离子浓度对人乳腺癌MCF-7细胞Caspase-3,Bax及Bcl-2表达的影响
- Author:
Jiangtao HAN
;
Zhenxiang YAO
;
- Publication Type:Journal Article
- Keywords:
BREAST NEOPLASMS/pathol;
[Ca 2+ ]i/blood;
GENE EXPRESSION
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of rising [Ca 2+ ]i on the expression of Caspase 3, Bax and Bcl 2 in human breast cancer MCF 7 cell lines. Methods After the MCF 7 cells treated with different dose of thapsigargin(TG), intracellular calcium concentrations in MCF 7 cells were measured by calcium sensitive fluorescence indicator Fura 2/AM technique; and immunohistochemical method was used to detected the expressions of Caspase 3, Bax and Bcl 2. Results Bax was over expressed and Bcl 2 was down expressed in a [Ca 2+ ]i concentration dependent way;nevertheless, the expression of Caspase 3 was negative in all the TG treated cells. Conclusions The increase of intracellular calcium concentration may have an inductive effect of apoptosis on MCF 7 cells, which is independent to Caspase 3.
