Isolation and purification of Thy-1~(low) Lin~- Sca-1~+ bone marrow stem subset cells by magnetic activated cell sorting
- VernacularTitle:免疫磁性分选骨髓干细胞亚群Thy-1~(low) Lin~- Sca-1~+
- Author:
Jingsong HE
;
Jisheng CHEN
;
Jun MIN
;
Jing WEI
;
Yunfeng CAI
;
Jing SHAO
- Publication Type:Journal Article
- Keywords:
BONE MARROW STEM SUBSET CELL;
MAGNETIC ACTIVATED CELL SORTING
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and purify Thy 1 low Lin Sca 1 + bone marrow stem subset cells by magnetic activated cell sorting(MACS). Methods Thy 1 low Lin Sca 1 + cells from mouse bone marrow were collected through three processes by MACS.After Lin + cells were removed,Thy 1 low Lin Sca 1 + bone marrow stem cell subsets were harvasted.The purity of the cells was analysed by FACS and the reclaimation rate was counted. Results The purity and reclaimation rate of Thy 1 low Lin Sca 1 + cells were 72.36% and 82.43% respectively, which equaled to the level of isolation and purification of CD34 + cells by MACS. Conclusions It is effective to isolate and purify Thy 1 low Lin Sca 1 + bone marrow stem cell subsets by MACS, and the purity and reclaimation rate of the cells are high.
