Biological effect of p27~(KIP1) on gastric carcinoma cells SGC7901 in nude mice
- VernacularTitle:外源性p27~(KIP1)基因对人胃癌细胞SGC7901的生物学效应
- Author:
Jianyong ZHENG
;
Weizhong WANG
;
Kaizong LI
;
- Publication Type:Journal Article
- Keywords:
STOMACH NEOPLASMS/genet;
GENES,SUPPRESSOR,TUMOR;
BIOLOGICAL EFFECT
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the biological effect of p27 KIP1 on gastric carcinoma cells SGC7901. Methods The total length of p27 KIP1 cDNA was transfected into human gastric cancer cells SGC7901 by lipofectin transfection. Expression of p27 KIP1 in protein or mRNA level was examined by Western blotting and RNA dot blotting respectively. Effect of p27 KIP1 on cell growth was observed by trpan blue exclusion assay. Tumorigenicity test in nude mice was applied to assess the biological effect of p27 KIP1 in vitro. Results Expression of p27 KIP1 in protein or mRNA increased evidently in SGC7901 cells transfected with p27 KIP1 . The cell growth was reduced by 42% about 48h after the induction with Zn 2+ ,which was determined by cell viability assay. The tumorigenicity of nude mice was reduced evidently(P
