The effect of bFGF on apoptosis of cultured human retinal pigment epithelial cells exposed to visible light
- VernacularTitle:碱性成纤维细胞生长因子对可见光诱导的人视网膜色素上皮细胞凋亡的影响
- Author:
Yongdong ZHOU
;
Mi YAN
;
Junjun ZHANG
;
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor,basic;
Pigment epithelium of eye;
Cell death;
Caspase
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of exogenous basic fibroblast growth factor (bFGF) on apoptosis of cultured human retinal pigment epithelial (RPE) cells exposed to visible light,and determine the role of bFGF, fibroblast growth factor receptor 1 (FGFR1),bcl 2 and caspase 3. Methods (2000? 500) lx cold white light was used. Exogenous bFGF was utilized during culture. Annexin annexin V fluorescein isothiocyanate/propidium iodium (V FITC/PI) labeling,flow cytometry, Immunocytochemical staining, enzyme associated absorb examing and reverse transcriptional polymerase chain reaction (RT PCR) were used to determine the apoptosis, the expression levels of bFGF, FGFR1, bcl 2, as well as the activity of caspase 3. Results No protective effect of bFGF was observed under the concentration 5 ng/ml. A significant inhibition of apoptosis was found in 10 ng/ml and 20 ng/ml groups ( P5 ng/ml) groups than light exposure groups ( P
