Nerve growth factor prevents apoptosis induced by indomethacin in cultured human fetal retinal pigment epithelial cells
- VernacularTitle:神经生长因子对吲哚美辛诱导人胚胎视网膜色素上皮细胞凋亡的保护作用
- Author:
Wensheng LI
;
Deyong JIANG
;
- Publication Type:Journal Article
- Keywords:
Nerve growth factor/pharmacology;
Pigment epithelium, eye;
Cell death;
Indomethacin;
Embryonic induction
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of nerve growth factor (NGF) on apoptosis of cultured human fetal retinal pigment epithelium (HFRPE) cells induced by indomethacin (IN) in vitro. Methods Subcultured HFRPE cells were treated with different concentrations of IN to establish apoptotic model. The protective effect of NGF on apoptosis of cultured HFRPE cells were assessed using an acridine orange (AO) staining method and transmission electron microscopy (TEM). Results HFRPE cells exposed by 200 600 ?mol/L IN for 24 hours elicited typical apoptosis morphological changes, including condensed chromation, nuclear fragmentation and reduction of nuclear size and cell volume. There was a statistically difference in HFRPE cells with apoptosis between 200 ?mol/L IN+500 ?g/L NGF and 200 ?mol/L IN groups ( q=3 9204,P=0.0320) ; there was a significant difference in HFRPE cells with apoptosis in 400 ?mol/L IN+500 ?g/L NGF and 400 ?mol/L IN as well ( q=9 7915,P=0 0001). Conclusion NGF has an protective effect on IN induced HFRPE cells apoptosis.
