The transfection of epidermal growth factor receptor antisense oligonucleotide to retinal glial cells
- VernacularTitle:表皮生长因子受体反义寡核苷酸转染人视网膜神经胶质细胞
- Author:
Mingshui FU
;
Xi ZHANG
;
Qinghua QIU
;
Qing GU
- Publication Type:Journal Article
- Keywords:
Neuroghia;
Retina;
Epidermal growth factor;
Oligonucleotides, antisense;
Gene therapy
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the permeability and stability of the transfection of antisense oligonucleotide (ASODN) hybridized epidermal growth factor receptor (EGFR) to retinal glial cells (RG). Methods Phosphorothioate and unmodified EGFR ASODN conjugated with 5′ isothiocyanate (5′ FITC) were encapsulated with or without lipofectin, and then added into human retinal glial cells culture media. The cellular permeability and stability of the transfection were observed by fluorescence microscopy in fixed cells. Results In the absence of lipofectin, phosphorothioate and unmodified EGFR ASODN were found in a few RG cells at 30 minutes, and in about 50% RG cells at 4 hours. Phosphorothioate EGFR ASODN were kept in RG cells for 3 4 hours and disappeared at about 8 hours. In the presence of lipofectin, phosphorothioate and unmodified EGFR ASODN were found in a few RG cells at 15 minutes and about 70% 80% RG cells at 4 hours. Phosphorothioate EGFR ASODN were kept in cells for 10 12 hours, and phosphorothioate and unmodified EGFR ASODN were disappeared at about 14 hours and 4 hours respectively. Conclusion 5′ FITC EGFR ASODN encapsulated with lipofectin could enter RG cells and express stably in RG cells.
