Construction of RhoC gene vector and its expression in eucaryotic cells
- VernacularTitle:RhoC基因真核表达载体的构建及其表达
- Author:
Yansong ZHENG
;
Xinsheng LU
;
Yongkun LIN
;
- Publication Type:Journal Article
- Keywords:
GENE RhoC;
GENE EXPRESSION REGULATION, NEOPLASTIC;
LIVER NEOPLASMS/genet
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an experimental model for exploring the role of RhoC gene in the invasiveness and metastasis of hepatocellular carcinoma.Method The RhoC gene was digested with restricted enzyme Hind III and XbaI,and direct cloned to pcDNA3.1.The recombinant vector (pcDNA3.1 RhoC) and the vector alone (pcDNA3.1) were transfected into HEPG2 cells with LIPFECTAMINETMReagent.After selected with hygromycin,resistant cloneies was obtained.The transcription and translation of RhoC gene were analysed with the reverse transcription PCR and immunohistochemical stain.Results The recombinant vectort (pc DNA3.1 Rhoc) express steadily in HerpG2 cells.Conclusions The modified tumor cells(HEPG2 RhoC) could be used to study the effect of RhoC protein on the invasiveness and metastasis of hepatocellular carcinoma.
