Prevention of abdominal adhesion with polyclonal antibody of transforming-growth factor beta : an experimental study
- VernacularTitle:转化生长因子-?多克隆抗体预防腹腔粘连的实验研究
- Author:
Yongqiang WENG
;
Yanyuan TU
;
Li XIAO
;
Xiaoyu LU
;
Jianxiong TANG
;
Aiqun CHENG
- Publication Type:Journal Article
- Keywords:
ABDOMINAL ADHESION/prev;
TRANSFORMING GROWTH FACTOR ?;
ANTIBODY POLYCLONAL
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To block the bio function of TGF ? by the application of anti TGF ? polyclonal antibody, and observe its efficacy of prevention of abdominal adhesion. Methods The postoperative abdominal adhesion model was established in SD rats. The drugs were administrated by abdominal injection with saline (control group), sodium hyaluronate( HA group), and varied dosage of anti TGF? (anti TGF? groups) respectively. The adhesion was scored 21days later, while 30 of them were executed on the day 3 and day 10 after operation respectively. The expression of TGF ? was checked by immunohistochemistry in the samples obtained from the adhesion sites. Results The score of adhesion in anti TGF ? group (2.4?0.99) was significantly lower than that in control group (6.0?1.25) and HA group (3.4?1.03); in different dosage of anti TGF, the 50?g group showed its economical efficacy; in the control and HA groups the expression of TGF ? had a time dependent manner, which reachs to maximum in the day 3, and could be reduced by antibody. Conclusions The polyclonal antibody of TGF ? shows the power to prevent the postoperative abdominal adhesion in animal model, the mechanism of which is due to inhibition of TGF ? expression.
