An experimental study on the neuron apoptosis and the axon changes of an acute brainstem injury
- VernacularTitle:大鼠急性脑干损伤神经细胞凋亡和轴突的变化
- Author:
Hua LIU
;
Jian LI
;
Li LIU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Brainstem injury;
Apoptosis;
neurofilament (NF)
- From:
Chinese Journal of Forensic Medicine
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study pathological changes of acute brainstem injury and evaluate their significance in forensic postmortem diagnosis of brainstem injury. Method To establish an acute brainstem injury model by using a gravitational force of a free falling weight. 50 rats were divided into two groups, an experimental group (35 rats) and a normal control group (15 rats): Neuron apoptosis and neurofilament (NF) of the brain stem were observed by HE, TUNEL and LSAB methods respectively. Results The brainstem injury model was mimic with that occurred in the forensic pathological practice. HE stain showed congestion, edema, loosening of the blood vascular wall as well as the circle hemorrhage of the brain stem and other part of the brain tissues. TUNEL method showed that the neuron apoptosis increased significantly in the cerebral cortex (P
