Effect of different anticancer drugs on the hyperplasia and telomerase activity of breast cancer cell MCF-7
- VernacularTitle:不同抗癌药物对乳癌MCF-7细胞增殖及端粒酶活性的影响
- Author:
Rongqing PANG
;
Buzhen ZHANG
;
Jian CHEN
;
Xinhua PAN
;
Zhilong CHEN
;
- Publication Type:Journal Article
- Keywords:
ANTINEOPLASTC AGENTS/pharm;
TELOMERASE;
CELL TRANSFORMATION,NEOPLASMS/drug eff;
;
BREAST NEOPLASMS;
TUMOR CELL,CUTURED
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the alteration of telomerase activity of MCF 7 cell line of breast cancer in the presence of different anticancer drugs. Methods The hyperplasia and viability of MCF 7 cell were detected by cell counting and trypan blue exclusion, and the telomerase activity was measured by TRAP.The alteration of MCF 7 cell and its related factors of telomerase activity were observed on cell growing in different condition. Results In abscence of drug, there was a positive correlation between hyperplasia and telomerase activity of the cell(r= 0.901 ). Adriamycin, paclitaxel and cisplatin could obviously inhibit the growth and reduce the telomerase activity of the cell in a dose-dependent and time-dependent fashion, and this reduction in telomerase activity closely correlated with the reduction in the number of viable cells. Conclusions Adriamycin, paclitaxel and cisplatin can inhibit the growth of MCF 7 cell, which may be correlated with the reduction in telomerase activity and cell viability.
