Experimental study of dendritic cells inducing immunoeffects on anti-gastric cancer
- VernacularTitle:树突状细胞诱导抗胃癌免疫效应的实验研究
- Author:
Xiao LEI
;
Yan SHI
;
Peiwu YU
;
Yun RAO
- Publication Type:Journal Article
- Keywords:
STOMACH NEOPLASMS/immunol;
DENDRITIC CELL;
ANTIGENS,NEOPLASM;
TUMOR CELL,CULTURED
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti tumor effects of cytotoxic T lymphocytes induced by tumor antigen specific dendritic cells. Methods Soluble tumor antigen was prepared by four freeze thaw cycles of gastric tumor cell line SCG7901. Anti gastric tumor vaccine was acquired by co incubation of tumor antigen and mouse bone marrow derived dendritic cells and cultured with granulocyte/macrophage colony stimulating factor and interleukin 4. Antigen specific cytotoxic T lymphocytes were induced by this tumor vaccine from the spleen and were applied to tumor bearing nude mice. Results Tumor growth was significantly inhibited and the apoptosis of tumor cells was promoted extensively. Conclusions Antigen specific dendritic cell tumor vaccine may play an important role in future immunotherapy of gastric cancer.
