The determination of 7-aminonitramepam, major metabolite of nitramepam, in urine by HPTLC
- VernacularTitle:高效薄层色谱法检测尿中硝西泮的代谢物7-氨基硝西泮
- Author:
Yuhong WU
;
Jiayi TAN
;
Ming CHENG
;
- Publication Type:Journal Article
- Keywords:
Nitrazepam;
7-aminonitrazepam;
Fluorescamine;
Thin-layer chromatography;
Urine
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A rapid and highly sensitive method, based on the high performance thin-layer chromatography, was described for the qualitative and semiquantitative determination of 7-aminon-itramepam, the main metabolites of nitramepam, in human urine. Method 7-aminonitramepam was extracted by diethyl ether under pH9 condition. Fluorecsamine was used as a reagent to produce fluorescent product to show analyte on plate. Results The limit of detection for 7-aminonitramepam in urine was 5ng/ ml and the limit of quantitation was 15ng/ml. Conclusion The method can be successfully used for determining 7-aminonitramepam in urine samples excreted over 48h period after receiving 10mg nitramepam o-rally and could be applied to drug examination for the cases of drug-facilitated rob.
