Effects of chronic hypoxia on expression of NOSⅢ mRNA induced by acute hypoxia in pulmonary artery endothelial cells
- VernacularTitle:慢性缺氧对肺动脉内皮细胞Ⅲ型一氧化氮合酶mRNA表达的影响
- Author:
Wei KONG
;
Dixun WANG
;
- Publication Type:Journal Article
- Keywords:
Anoxia;
Nitric oxide synthase;
Endothelium;
Cells
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS: In situ hybridization and image-analysis system were used to investigate the expression of NOSⅢ mRNA in cultured porcine pulmonary artery endothelial cells (PAEC) under normoxic or chronic hypoxic conditions. RESULTS: The expression of NOSⅢ mRNA were increased by 48%, 125%, 119% respectively in the 2nd, 4th, 6th subculture of normoxic PAEC after expouse to acute hypoxia for 12 hours while in chronic hypoxic group,the expression of Ⅲ NOS mRNA were increased by 92%, 245%, 565% separately. The increase was more apparent in chronic hypoxic group than in normoxic group and became more significant following the prolonging duration of hypoxia. CONCLUSION: Chronic hypoxia increased the expression of NOSⅢ gene in PAEC in response to acute hypoxia. Nitric oxide may play an important role in the decrease of hypoxic pulmonary vasoconstriction caused by chronic hypoxia.
