Molecular Determination of Dermato phyte Fungi Using Arbitrarily Prime d Polymerase Chain Reaction
- VernacularTitle:用任意引物聚合酶链反应进行皮肤癣菌的子生物学研究
- Author:
Xiangming KONG
;
Guoling YANG
;
Liji JIN
;
- Publication Type:Journal Article
- Keywords:
Dermatophyte;
Arbitrarily primed p olymerase chain reaction
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the significance of AP-PCR i n identification and subtyping of der-matophytes.Methods Using a pair of random primers,OPAA11(5' -ACCCGACCTG -3' ),and OPD18(5' -GAGAGCCAAC -3' )the DNAs of 64isolates of dermatophytes(9species of 3genera),Sporothrix schenckii and Candida albicans were amplified by AP-PCR and analyse d by electrophoresis.Results Distinct DNA band patterns were observed in diffe rent dermatophyte species.Common major DNA bands were observed in Trichophyton rubrum isolated from different areas with s train difference.Conclusion Using OPAA11and OPD18as primers,AP-PCR may be applied in the identification and subtypi ng of dermatophytes.
