Damag to gut mucosal barrier and intestinal bacteria-endotoxin translocation in acute necrotizing pancreatitis rats
- VernacularTitle:急性坏死性胰腺炎时肠屏障损害及肠源性细菌和内毒素移位的实验研究
- Author:
Qun DENG
;
Zhanliang LI
;
Lianrong LU
;
Yanjie LIANG
;
Xiaoqing SUN
;
- Publication Type:Journal Article
- Keywords:
PANCREATITIS/physiopathol;
?INTESTINAL MUCOSA/physiopathol;
?LACTATES/blood;
?ENDOTOXINS/blood;
?BACTERIA, TRANSLOCATION;
?ENDOTOXIN, TRANSLOCATION
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes in gut mucosal barrier and gut-origin bacteria-endotoxin translocation in acute necrotizing pancreatitis (ANP) rats. Methods Wistar rats were divided randomly into normal group (n=6), sham operation group (n=30) and ANP group (n=39). ANP was introduced by infusion of artificial bile into biliopancreatic duct. Morphology of pancreas and intestine were observed and tight junction on ileum epithelia were assessed by cryofracture replicas electroscopy. Plasma levels of D-lactic acid and endotoxin were examined at various time points. The rates of bacterial translocation to abdominal organs were also calculated. Results Mucosal and tight junction damages of the gut were found during early stage of ANP. Simultaneously, plasma D-lactate levels increased and endotoxemia occurred. The rate of bacterial translocation to organs was 59.5% 72h after ANP occurred. Conclusions Gut barrier function can be injured in the early stage of ANP, and resulting in gut origin bacteria-endotoxin translocation, which may be the originator of systemic inflammatory reaction and secondary infection of the pancreas.
