Effect of mild hypothermia on the expression of the inducible HSP70 in canine brain tissue after globai cerebral ischemia reperfusion
- VernacularTitle:浅低温对犬全脑缺血再灌注后脑组织诱导型热休克蛋白70表达的影响
- Author:
Fenghua LI
;
Xiujuan TAN
;
Hongwei CAI
;
Zhigang CHENG
;
Qulian GUO
;
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Hypothermia,induced;
Resuscitation;
Heat-shock proteins 70;
Brain
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechnism of mild hypothermia during cerebral resuscitation. Method:Fourteen dogs were anaesthetized with ketamine and fentanyl and were divided randomly into three groups. Four dogs as nonischemic control(group A).recived general care for 24 hours after operation without cardiac arrest. The other ten dogs underwant a 18-minute cardiac arrest.were resuscitated with standard CPR.and supported by intensive care for 24 hours,then divided into two groups. Five dogs were treated with routine cerebral resuscitation measures (RCRM) (group B):five dogs were treated with RCRM and mild hypothermia started 10 min after CPR and mantained at 34 C?0.5 C for 12 hours (group C). At the end of experiment,the parietal cerebral cortex was obtained to detect the expression of heat shock proteins with immunohistochemical technique and to observe the change of histomorphology. Result: The pathologic change in group C was more obviously mitigated than that in group B. The HSP70-LI positive neuronal density and grey values in group B and C were more significantly increased than those in group A (P
