Experimental Study of Antifungal Effect of Povidone Iodine Solution on Candida Albicans
- VernacularTitle:聚维酮碘溶液抗白念珠菌的实验研究
- Author:
Yuping RAN
;
Chaowu ZHANG
;
Rioji TSUBOI
;
- Publication Type:Journal Article
- Keywords:
Povidone iodine;
Candida albieans;
Keratinocytes
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The effect of povidone iodine solution(PIS)on the metabolic activity of Candida albi- cans and the effect of PIS treated C.altn'cans to cultured human keratinocytes were studied by using the Alamar blue fluorescence assay.It was found that PIS inhibited the metabolic activity of Candida albi- cans with a dose-dependent manner,and that the LD_(50)of PIS for Candida albicans is 0.075%.After treating for 30 min by 2% PIS/PBS(-),Candida albicans almost lost the adherence and invasion to the cultured human keratinocytes,the wall of yeast cells became uneven and shrunken,the intracellular structures became obviously indistinct,and the yeasts could not develop to hypha form.Candida albi- cans,treated with 2% PIS in PBS(-)for 30 min or with 10% PIS Sabouraud's liquid medium for 33 hr,did not form colony when it was inoculated to Sabouraud's dextrose agar.This result suggests that the fungicidal effective of PIS is influenced by its solvent.
