IMMUNOCYTOCHEMICAL STUDY WITH ANTI-MUSCLE ACTIN ANTIBODY(HHF_(35))ON MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY IN RATS
- VernacularTitle:大鼠心肌缺血再灌流损伤的免疫组化研究
- Author:
Chenzhong FU
;
Yixuan SONG
;
Guanhua XUE
;
Jiazhen ZHU
;
Qiming BI
;
- Publication Type:Journal Article
- Keywords:
Myocardium ischemia;
reperfusion injury;
Arrhythmias;
Anti-muscle actin antibody;
Immunocytochemistry
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Experimental studies on the myocardial ischemia and reperfusion injury in 16 anaethetized SDrats,of which,8 animals were pretreated with morphine(5 mg/kg,i.p.)for preventing of arrhyth-mias,were studied immunocytochemically with anti-muscle actin specific monoclonal antibody (HHF_(35)),8 shan-operated rats were used as control.With HHF_(35) ABC immunocytochemical method,the area of myocardial ischemia and reperfusion injury(without morphine)showed decrease or ab-sence of staining,large areas of staining loss were also seen.In the group with morphine,only smallfoci of staining absence were shown.The myocardium in control animals showed evenly positive stain-ing.No change were seen with HE staining in all groups.The results obtained with HHF_(35) stainingsupport its important value in studying on myocardic reperfusion injury,and indicated that the degreeof myocardic damage may be relative to the arrhythmias in myocardial reperfusion injury.
