AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS ANALYSIS OF P33.6 LOCUS IN CHINESE
- VernacularTitle:中国人p33.6位点的扩增片段长度多态性
- Author:
Song CHEN
;
Boling LI
;
Jintang NI
;
Lan HU
;
Anquan JI
;
Jian YE
- Publication Type:Journal Article
- Keywords:
p33.6 locus Polymerase Chain Reaction(PCR) Variable Number of Tandem Repeat(VNTR) Amplified Fragment Length Polymorphism(Amp—FLP)
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The polymorphisms of minisatellite p33.6 (D1S111) locus were typed rapidly and accurately us- ing polymerase chain reaction (PCR). The amplified fragments were analyzed by mini- polyacrylamide gel electrophoresis followed by silver stain. The distribution of allele frequencies among 100 unrelated Chinese is reported. 14 alleles, containing 9 to 22 repeat units, had been detected with a heterozygosity of 76%. The allele frenquencies were from 0.5 %to 35.5 %. The size of amplified fragments ranged from 435 bp to 925bp. The discrimination power of p33.6 locus was 0. 916. The Amp --FLP was inherited according to the Mendelian Law. The results showed that the polymorphisms of p33.6 locus can be used for individual identification and paternity test.
