PREPARATION AND PRACTICAL APPLICATION OF MONOCLONAL ANTI-A ANTIDODIES
- VernacularTitle:抗人A型红细胞单克隆抗体的研制及初步应用
- Author:
Yawei ZOU
;
Jingyuan GUO
;
Yuelong CHEN
;
- Publication Type:Journal Article
- Keywords:
A blood group Monoclonal anti-A antibodies Hybridoma solid tumor
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Balb/c mice were immunized by intraperitoneal iniection of group A erythrocytes. Immunized spleen cells were fused with Sp2/0 murine myeloma cells from the solid tumour. After 10～15 days of incubation at 37℃ CO_2, the supernatants were screened for the agglutinabal antibodies. Three hybridoma cell lines secreting high titer and specific monoclonal anti-A antibodies were established.These hybridoma cells have the ability of constant seceting antibodies which belong to the IgM class. The specificity monocloal anti-A sera was the same as the human anti-A sera.
