PREPARATION AND APPLICATION ON MONOCLONAL ANTI-A ANTI-B ANTIBODIES
- VernacularTitle:人类A、B抗原单克隆抗体的研制和初步应用
- Author:
Peizhong WEN
;
Yingsong KUAI
;
Xiangju WANG
;
Xiulan XU
;
- Publication Type:Journal Article
- Keywords:
ABO blood group Monoclonal antibody Hybridoma Titer
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
BALB/C mice were immunized by intraperitoneal injection with A-group erythrocytes, B-group erythrocytes and saliva of A-group,B-group secretor. Immune spleen cells were fused with sp2/0 murine myeloma cells. The supernatants from each well were examined for antibodies by hemagglutination using A-,B-, O-group RBC.3 hybridoma cell lines of secreting anti-A antibodies and 3 hybridoma cell lines of secreting anti-B antibodies were established.These hybridoma cells have maintained and produced antibodies possessing higher specificity and titer.The agglutination titer of supernatants reached 2048-fold. These antibodies were used in the field of forensic science.
