IDENTIFICATION OF DEATH CAUSED BY SNAKE BITING BY ABC-IMMUNOCYTOCHEMICAL TECHNIQUE
- VernacularTitle:用ABC法鉴定眼镜蛇中毒死亡的实验研究
- Author:
Jianmin ZHANG
;
Jiazhen ZHU
;
- Publication Type:Journal Article
- Keywords:
Snake-bite;
Venom;
Immunocytochemistry technigue;
Forensic medicine
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Varying doses of cobra crude venom were injected subcutaneously into rats.Formaking frozen and methylaldehyde fixed,paraffin embeded sections,tissues blockswere taken from the injected site muscles,hearts,livers,spleens,lungs,kidneysand diaphragms either immediately,or 6,12,24hrs after death.Tissue sectionswere stained with and ABC-immunocytochemical techniques.On HE stained sections,edema,heamorrhage,congestion were observed.Coludyswelling,necrosis,and breakdown of cells were seen in most of the organs.Nospecific morphological changes has been found.On the frozen sections of the musc-les taken from the injected site,kidneys and livers,the venom has been identifiedby ABC-immunocytochemical staining.Although as little as 2LD_(50) (2mg/kg) venomwas given,it could be demonstrated on the membrane of the muscle even 24 hrs afterdeath.In case of large amount of venom (11LD_(50) ) poisoning,the venom was alsofound in nucleus of liver cells and in the endothelial cells of capillaries of renalglomeruli.The pathogenesie of localization of snake venom in endothelial cells ofrenal glomerular capillaries was discussed,there is No report about the localizationof snake venom in nucleus of liver cells It is a very interesting phenomenonIt is usggested that the ABC-immunocytochemical stain is a sensitive and specificmethodforid entifying sanke venom in tissues of snake biting victims.
