STUDIES ON RP-HPLC FOR THE SIMULTANEOUS DETERMINATIONS OF CHLORPROMAZINE AND CLOZAPINE IN HUMAN SERUM AND CLINICAL APPLICATIONS
- VernacularTitle:反相高效液相色谱法同时测定人血清中氯丙嗪与氯扎平的浓度
- Author:
Aiqing ZHANG
;
Lingmei CHEN
;
Qiying ZHANG
;
Zhiling QIN
;
- Publication Type:Journal Article
- Keywords:
Chlorpromazine Clozapine Reversed-phase high performance liquid chromatographic method Determination of drug serum levels
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
1ug/ml),chromatographing ona 300?3.9mm 1.D.colomn packcd with u Bondapak C_(18)(10um),and detecting at UV254nm.The mobile phase was MeOH-H_2O-TEMED-HAC(65:35:0.3:0.52)The average recovery of the whole procedure were 99.9?4.1(SD)%.CV=4.1%for clozapine)98.5?4.0(SD)%,CV=4.2% for chlopromazine.The Minimum detetionlimit was 10ng/ml serum.The developed method was applied to clinical monitoring of serum levels from11 patients who wereco administered with multiple oral doses of clozapine and chlor-promazine.The serum concentrations of 0.150-0.445?g/ml and 0.014-0.086?g/ml werefound.Futhermore,one case of suicide was encountered.
