PREPARATION AND IDENTIFICATION OF ANTI-GC SERUM
- VernacularTitle:抗GC血清的制备及鉴定
- Author:
Yiping HOU
;
Meiyun WU
;
- Publication Type:Journal Article
- Keywords:
Group specific component Vitamin D binding protein GC protein Anti- GC serum
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Anti-GC serum was successfully prepared in two New Zealand rabbits immunized with GC protein which was isolated and purified from GC2-2 serum previously in our laboratory. The results of identification showed that the specificity of the home made anti- GC serum and the commercial anti- GC serum (DAKOPATTS) were identical. The titer of the home made anti-GC serum was 128. Three common phenotypes, GC1-1, GC2-1 and GC2-2could be identified by immunoelectrophoresis with the home made anti-GC serum. The concentration of GC protein as low as 3.1 ?g/ml could be detected by double immunodiffusion. In addition the anti-GC serum does not cross react with other human serum proteins.
