THE RAPID DETERMINATION OF ABO BLOOD GROUP IN BODY FLUIDS (OR STAINS)BY DOT-ELISA USING ENZYME-LABELLED MONOCLONAL ANTIBODY
- VernacularTitle:应用斑点ELISA快速进行体液(斑)的血型测定
- Author:
Bin ZHOU
;
Jingyuan GUO
;
Chuanxi WANG
;
Jian CHEN
;
- Publication Type:Journal Article
- Keywords:
ABH;
enzyme-labelled;
McAb;
Dot-ELISA;
ABO blood group;
Body fluid stains
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
One step dot-ELISA method for the rapid determination of ABO blood groups in humam body fluids(or stains)was established using enzyme-labelled anti-A,--B and anti--H monoclonal antibody (McAb).The sample was applied on the nitrocellulose membrance as a dot. After washing, the appropriate McAb wasadded on top of the dot, and then followed by adding 3, 3'-diaminobenzidine(DAB). The brown color indi-cated the positive reaction. The ABO blood typing of 521 saliva samples including both secretor and non-se-cretor were carried out. All the results were correct. The advantages of this method are accurate, sensitive,rapid, easy to perform, as well as not time consuming. It is more sensitive than the conventional hemaggluti-naton inhibition test.
