Determination of methaqualone and 2-methyl-3[2-(hydroxymethyl) phenyl]-4 (3H)quinazolinone concentrations in human serum by RP-HPLC
- VernacularTitle:高效液相色谱法测定人血清中安眠酮及其苯甲基羟化代谢物的浓度
- Author:
Qiuming CHU
;
Aiqin ZHANG
;
Qiying ZHANG
;
- Publication Type:Journal Article
- Keywords:
RP-HPLC Methaqualone 2-methyl-3 [2-(hydroxymethyl) phenyl]-4(3H) quinaxolinone
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Methaqualone and 2-methyl-3 [2- (hydroxymethyl) phenyl]- 4 (3H) quinazolinone (metabolite I) concentrations in human serum wereanalyzed quantitatively by reverse high performance liquid chromatography (RP-HPLC). The concentrations of methaqualone ranging from 1-35?g/ml were in good linear relationship(Correlation coefficient r = 0. 9980, regression line y = 0. 06324x-0. 1029), The average recovery of methaqualone reached as high as 102.08?9. 987(SD)% (n= 5). The detective limit was 1 ng. The relative quantity of metabolite I was determined according to the linear concentration of methaqulone. RP-HPLC assay is proved to be useful in determening blood methaqualone and metabolite I concentrations in forensic toxicology.
