Anti-diabetic effects of Caulerpa lentillifera：stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes
10.12980/APJTB.4.2014APJTB-2014-0091
- Author:
Sharma Raj Bhesh
;
Rhyu Young Dong
- Publication Type:Journal Article
- Keywords:
Caulerpa lentillifera;
Diabetes;
Glucose uptake;
Insulin secretion;
RIN cells;
3T3-L1 adipocytes
- From:Asian Pacific Journal of Tropical Biomedicine
2014;(7):575-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera).Methods:The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV andα-glucosidase enzyme was measured in a cell free system. Then, interleukin-1β and interferon-γinduced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes.Results: C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and α-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1βand interferon-γ induced RIN cells. Furthermore, C. lentillifera extract significantly enhanced insulin secretion in RIN cells and glucose transporter expression and glucose uptake in 3T3-L1 adipocytes.Conclusions:Thus, our results suggest that C. lentillifera could be used as a potential anti-diabetic agent.
