Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin
10.1016/S2221-1691(14)60236-7
- Author:
Febriansah Rifki
;
PP Dewi Dyaningtyas
;
Nurulita Aries Nunuk
;
Meiyanto Edy
;
Nugroho Endro Agung
- Publication Type:Journal Article
- Keywords:
Hesperidin;
Doxorubicin;
MCF-7/Dox cells line;
Apoptosis;
Pgp expression
- From:Asian Pacific Journal of Tropical Biomedicine
2014;(3):228-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin.
Methods:The cytotoxic properties, 50%inhibition concentration (IC50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp.
Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC50 value of 11 μmol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI>1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration.
Conclusions: Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC50 of 11 μmol/L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Co-chemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression.
