Recovery and Adsorption Rate of Murine Norovirus Using NanoCeram(R) Filters.
- Author:
Yun Hee KIM
1
;
Seok Jea JANG
;
Ji Youn PARK
;
Jung Hwan OH
;
Geun Su KIM
;
Tae Seung KIM
;
Oh Sang KWON
;
Jin Seok HAN
;
Won Hwa JHEONG
Author Information
- Publication Type:Original Article
- Keywords: NanoCeram(R) filter; Norovirus; Murine norovirus; Recovery; Adsorption
- MeSH: Absorption; Adsorption; Humans; Norovirus
- From:Journal of Bacteriology and Virology 2011;41(1):55-61
- CountryRepublic of Korea
- Language:Korean
- Abstract: This study investigated the recovery and absorption rates of murine norovirus, a surrogate for human norovirus, by using NanoCeram(R) filters which served as a tool for recovering viruses. In the study, two types of NanoCeram(R) filters were employed: one was a cartridge type and the other was a disc type (phi 47 mm) whose surface area is 75 times smaller than the cartridge type. The analytical method was the real-time reverse transcription-polymerase chain reaction (RT-PCR). The study found that the average recovery rates of the cartridge type and the disc type were 30.9% and 29.5% respectively. Since these two rates were very close to each other, the adsorption rate of the cartridge type could be predicted with the disc type. Analyzing recovery and absorption rates of the disc type based on different filtered volumes showed that when the volume increased from 0.5 L to 20 L, the average recovery rate rose from 14.78% to 30.41 %, while the average absorption rate dropped from 56.33% to 10.48%. The increase in turbidity from less than 1 NTU to less than 3 NTU raised the average recovery rate from 47.23% to 82.84%.
