Cytotoxicity and genotoxicity assessment of Euphorbia hirta in MCF-7 cell line model using comet assay
10.1016/S2221-1691(13)60140-9
- Author:
Ping Yuet Kwan
;
Darah Ibrahim
;
Chen Yeng
;
Sasidharan Sreenivasan
- Publication Type:Journal Article
- Keywords:
Euphorbia hirta;
Cytotoxicity;
Genotoxicity;
Cell Line Model
- From:Asian Pacific Journal of Tropical Biomedicine
2013;(9):692-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta (E. hirta) in MCF-7 cell line model using comet assay.
Methods: The cytotoxicity of E. hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E. hirta was assessed by using Comet assay.
Results: Both toxicity tests exhibited significant toxicity result. In the comet assay, the E. hirta extract exhibited genotoxicity effects against MCF-7 DNA in a time-dependent manner by increasing mean percentage of DNA damage. The extract of E. hirta showed significant toxicity against brine shrimp with an LC50 value of 620.382 μg/mL (24 h). Comparison with positive control potassium dichromate signifies that cytotoxicity exhibited by the methanol extract might have moderate activity.
Conclusion:The present work confirmed the cytotoxicity and genotoxicity of E. hirta. However, the observed toxicity of E. hirta extracts needs to be confirmed in additional studies.
